Research Paper Volume 12, Issue 10 pp 9103—9124

A novel 3’,5’-diprenylated chalcone induces concurrent apoptosis and GSDME-dependent pyroptosis through activating PKCδ/JNK signal in prostate cancer

Figure 9. C10 attenuated tumor growth by inhibiting cell proliferation and inducing apoptosis and inflammation in PC3 xenograft mice. (A) PC3 cell tumor xenograft nude mice were intraperitoneally administered C10 (low-dose or high-dose) or the control treatment every two days for a total of 10 times, as indicated in the diagram. (B) The tumor sizes in the three groups were monitored and recorded at three-day intervals as soon as C10 was injected. (C) The subcutaneous tumors were weighed immediately at the end of the study. (D) The mouse weights in the three groups were recorded at three-day intervals as soon as C10 was injected. (E) Tumor spheroids generated from the control and high-dose groups were fixed, sectioned and immunohistochemically stained for PKCδ, PCNA, Bcl-2 and IL-6 expression. The levels of the indicated proteins were quantified in the control and high-dose groups (Scale bar: 50 μm). Data are shown as the mean ± SD. *P < 0.05, **P < 0.01.