Figure 4. Overexpression of Klotho suppresses the IS-induced inflammatory response in vivo by promoting M2 macrophage polarization. (A) The total IS concentration (mg/L) was measured by UPLC. (B–E) The levels of IL-1β, TNFα, IL-6, and IL-10 in serum from mice were analyzed by ELISA. (F) Representative FACS plots for M0 (F4/80+), M1 (F4/80+MMR-), and M2 (F4/80+MMR+) macrophages. The percentages of F4/80+, F4/80+MMR-, and F4/80+MMR+ cells were evaluated in kidney tissue using FACS. (G) The percentages of F4/80+, F4/80+MMR-, and F4/80+MMR+ cells were evaluated in heart tissue using FACS. (H) Quantification of relative expression based on F4/80, Klotho, and DAPI staining. *P < 0.05, **P < 0.01.