Research Paper Volume 12, Issue 13 pp 12771—12782

HOTAIR expands the population of prostatic cancer stem-like cells and causes Docetaxel resistance via activating STAT3 signaling

Figure 1. HOTAIR induction in PCa cells leads to increased population of cancer stem cells. (A) TCGA dataset showed that HOTAIR was highly expressed in metastatic CRPC samples. (B) HOTAIR overexpression in C4-2 cells (top) and Du145 cells (bottom) promoted the expression levels of several cancer stem cell markers. Gene expression was normalized to GAPDH mRNA. (C) HOTAIR overexpression in C4-2 cells and Du145 cells increased PCSLCs population. Top, Representative images of tumorspheres. Bottom, statistical analyses. (D) flow cytometry to analyze the population of CD133 positive cells before and after HOTAIR expression. (E) a positive correlation of HOTAIR and Oct4 was observed based on TCGA dataset. *p<0.05; **p<0.01.