Research Paper Volume 12, Issue 10 pp 9224—9239

Apelin enhances IL-1β expression in human synovial fibroblasts by inhibiting miR-144-3p through the PI3K and ERK pathways

Figure 2. APLN stimulates IL-1β expression in OASFs in concentration- and time-dependent manners. (A) Human OASFs were incubated with 0, 1, 3, and 10 ng/mL of APLN for 24 h, and IL-1β mRNA expression levels were examined by RT-qPCR (n=4). (B) OASFs were incubated under various concentrations of APLN for 24 h, and IL-1β expression levels were examined by Western blot (n=3). (C) OASFs were cultured under various concentrations of APLN for 24 h, and excreted IL-1β were examined by ELISA assay (n=5). (D) OASFs were incubated with 10 ng/mL of APLN for 0, 6, 12, and 24 h. IL-1β mRNA levels were examined by RT-qPCR (n=4). (E) IL-1β protein synthesis levels were examined by Western blot (n=3). (F) Excretion of IL-1β protein levels in human OASFs was examined by ELISA (n=5). * p<0.05 compared with control group.