Research Paper Volume 12, Issue 21 pp 21091—21113

MEX3A is upregulated in esophageal squamous cell carcinoma (ESCC) and promotes development and progression of ESCC through targeting CDK6

Figure 2. MEX3A knockdown inhibited development of ESCC in vitro. (A) The knockdown of MEX3A in Eca-109 and TE-1 cells was verified by western blotting. (B) MTT assay was performed to examine the effects of MEX3A knockdown on cell proliferation of Eca-109 and TE-1 cells. (C) The effects of MEX3A knockdown on the ability of Eca-109 and TE-1 cells to form colonies were evaluated by colony formation assay. (D) Flow cytometry was used to detect cell apoptosis of Eca-109 and TE-1 cells cells with or without MEX3A knockdown. (E) The differentially expressed apoptosis related proteins identified in Eca-109 cells with or without MEX3A knockdown by Human Apoptosis Antibody Array. (F, G) Cell migration ability of Eca-109 and TE-1 cells with or without MEX3A knockdown was detected by wound healing assay (F) and Transwell assay (G). The figures are representative data from at least three independent experiments. The data were expressed as mean ± SD (n ≥ 3), *P<0.05, **P<0.01, ***P<0.001.