Figure 6. CDK6 knockdown alleviated the effects of MEX3A overexpression on ESCC. (A) The expression of MEX3A and CDK6 in Eca-109 cells with simultaneous MEX3A overexpression and CDK6 knockdown was detected by qPCR and compared with negative control. (B) The change of expression of MEX3A and CDK6 in MEX3A+shCDK6 group of Eca-109 cells was visualized by western blotting. (C–F) The results of Celigo cell counting assay (C), colony formation assay (D), flow cytometry (E), wound-healing assay (F) and Transwell assay (G) showed that the effects of MEX3A overexpression on cell proliferation, colony formation, cell apoptosis and cell migration could be alleviated by CDK6 knockdown.