Research Paper Volume 12, Issue 21 pp 21091—21113

MEX3A is upregulated in esophageal squamous cell carcinoma (ESCC) and promotes development and progression of ESCC through targeting CDK6

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Figure 6. CDK6 knockdown alleviated the effects of MEX3A overexpression on ESCC. (A) The expression of MEX3A and CDK6 in Eca-109 cells with simultaneous MEX3A overexpression and CDK6 knockdown was detected by qPCR and compared with negative control. (B) The change of expression of MEX3A and CDK6 in MEX3A+shCDK6 group of Eca-109 cells was visualized by western blotting. (CF) The results of Celigo cell counting assay (C), colony formation assay (D), flow cytometry (E), wound-healing assay (F) and Transwell assay (G) showed that the effects of MEX3A overexpression on cell proliferation, colony formation, cell apoptosis and cell migration could be alleviated by CDK6 knockdown.