Research Paper Volume 12, Issue 21 pp 21091—21113

MEX3A is upregulated in esophageal squamous cell carcinoma (ESCC) and promotes development and progression of ESCC through targeting CDK6

Figure 6. CDK6 knockdown alleviated the effects of MEX3A overexpression on ESCC. (A) The expression of MEX3A and CDK6 in Eca-109 cells with simultaneous MEX3A overexpression and CDK6 knockdown was detected by qPCR and compared with negative control. (B) The change of expression of MEX3A and CDK6 in MEX3A+shCDK6 group of Eca-109 cells was visualized by western blotting. (CF) The results of Celigo cell counting assay (C), colony formation assay (D), flow cytometry (E), wound-healing assay (F) and Transwell assay (G) showed that the effects of MEX3A overexpression on cell proliferation, colony formation, cell apoptosis and cell migration could be alleviated by CDK6 knockdown.