Research Paper Volume 12, Issue 9 pp 8710—8727

Soy isoflavones improve the oxidative stress induced hypothalamic inflammation and apoptosis in high fat diet-induced obese male mice through PGC1-alpha pathway

Figure 7. Soy isoflavones increase PGC1-alpha activity in hypothalamic cell line. (A) Quantification shows the mRNA levels of PGC1-alpha, SOD2 and GPx were increased under daidzein(50μM) and genistein(50μM) treatment for 24h in N42 cells; (B, C) Western blots and quantification show the protein levels of PGC1-alpha, SOD2 and GPx were increased under daidzein(50μM) and genistein(50μM) treatment for 24h in N42 cells; (DF) Western blots and quantification show the SR-18292(20μM) administration inhibited the protein levels of SOD2 and GPx induced by daidzein and genistein treatment in N42 cells. (G, H) Western blots and quantification show the PA(100μM) administration inhibited the protein levels of PGC1-alpha, SOD2 and GPx, while daidzein and genistein could abolish the inhibition of PA in N42 cells. ns, no statistical significance, * p < 0.05, ** p < 0.01, ***p < 0.001.