Figure 4. Upregulated expression of PRMT5 promoted the proliferation and aggressiveness of bladder cancer cells. (A) Western blotting showed successful overexpression of PRMT5 in TCCsup cells. (B, C) Cell proliferation was promoted by the CCK-8 assay and the colony formation assay. (D, E) Cell invasion and migration of bladder cancer cells were detected using the transwell assay and the wound-healing assay. Error bars show the standard error of the mean. *P < 0.05, **P < 0.01.