Figure 5. BRD4 is essential for maintaining the chromatin environment for LPS-induced macrophage senescence. (A) To demonstrate the binding of the indicated proteins to the target genes in THP-1 macrophages, a heatmap was generated using the ChIP-qPCR values. ChIP-qPCR enrichments of no-antibody control (NA) BRD2, BRD3, BRD4, H3K27ac, and H3 using primers spanning –1 kb to +1 kb of the indicated SASP genes were arrayed from blue (no enrichment) to yellow (maximal enrichment). (B) The control H3 protein co-precipitated evenly with the ChIP targets. A heatmap was generated using the ChIP-qPCR values for histone H3 between –1 kb and +1 kb of the indicated genes. No significant changes were observed in H3 enrichment for the listed genes in each treatment group. P-values were determined using one-way ANOVA. (C, D) The representative ChIP-qPCR values for the IL-6 and CXCL1 genes selected from the experiments. (E, F) Representative ChIP-qPCR values for the IL-6 and CXCL1 genes selected from experiments with different treatments. The horizontal dotted line indicates the upper limit of the 95% confidence interval of the signal from no-antibody (NA) ChIP. Values are presented as the mean ± SEM of three independent experiments. *p <0.05 from one-way ANOVA.