Research Paper Volume 12, Issue 10 pp 9515—9533

Clioquinol improves motor and non-motor deficits in MPTP-induced monkey model of Parkinson’s disease through AKT/mTOR pathway

Figure 3. CQ improved the pathology of monkeys in the SN after MPTP intoxication. (A) Representative images of Nissl and immunofluorescence staining of TH (biomarkers of DA neurons) showed cell loss and cell atrophy in the SN after long-term of MPTP injection, and which were mitigated by both LD and CQ treatment. (B) Stereology analysis of cell count of DA neurons in the SN. (C, D) Western bolts and quantification showed increased protein expression of TH, DAT, and D3R in the SN of MPTP+LD and MPTP+CQ group in comparison to MPTP group. (E) Quantification showed the mRNA level of TH, DAT, and D3R in each group. (F, G) Western bolts and quantification showed increased protein expression of NF-M and MBP in the SN of MPTP+LD and MPTP+CQ group in comparison to MPTP group. Data expressed as the mean ± SD. *P<0.05, **P<0.01, indicate significant difference. Scale bar=200μm.