Research Paper Volume 12, Issue 10 pp 9633—9657

MicroRNA-214-3p targets the PLAGL2-MYH9 axis to suppress tumor proliferation and metastasis in human colorectal cancer

Figure 5. PLAGL2 accelerates CRC cell growth and migration. (A) WB indicated that downregulation of PLAGL2 could inhibit the expression of vimentin and N-cadherin and increase the expression of Zo-1 and E-cadherin. (BC) CCK8 assays indicated that PLAGL2 promoted the proliferation of CRC cells. (DE) Colony-formation assays indicated that PLAGL2 promoted the proliferation of CRC cells. (F) EDU assays indicated that PLAGL2 promoted the proliferation of CRC cells. (GH) Transwell and wound-healing assays showed that PLAGL2 promoted the migration and invasion of CRC cells. The data are represented as the means±S.D. from at least three independent experiments. *p<0.05.