Figure 4. The discrepancy between eggs ovulated and CLs tracked is partially accounted for by regressing CLs from previous estrous cycles. (A) The number of CLs tracked per ovary and the number of eggs ovulated per oviduct for reproductively young mice (N = 5 mice, 10 ovaries). (B) The number of CLs tracked per ovary and the number of eggs ovulated per oviduct for reproductively old mice (N = 5 mice, 10 ovaries). (C) Representative image of Galectin-3 IHC in an ovary from a reproductively young mouse. All CLs are outlined in blue. Galectin-3 positive CLs are indicated with black asterisks. The inset shows the IgG control. The scale bar is 100 μm. (D) Graph showing the percent of Galectin-3 positive CLs (~24.3%) and Galectin-3 negative CLs (~75.7%) (N = 20 ovaries, 3 slides per ovary). (E) The graph shows the average discrepancy between CLs and eggs for both age cohorts. A t-test was performed and there was no difference in the discrepancy between the age cohorts (P > 0.05).