Research Paper Volume 12, Issue 10 pp 9745—9760

Loss of macroH2A1 decreases mitochondrial metabolism and reduces the aggressiveness of uveal melanoma cells

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Figure 5. KD for macroH2A1 reprograms energy metabolism in UM 92.1 cells. (A) mRNA levels MT-ND4, MT-CO2, COX4|1, MT-CYB, ATP5F1A and TFAM were assessed by qPCR, and normalized to GAPDH levels. Values are presented as mRNA relative levels and they are expressed as the mean ± SEM of three different experiments. (p < 0.0001 vs control). (B) mRNA levels NMNAT1, NMNAT2, SIRT1 and NAMPT. Values are presented as mRNA relative levels and they are expressed as the mean ± SEM of three different experiments. (**p<0.01****p < 0.0001 vs control). (C) Representative immunocytochemical images showing staining for TFAM (upper panels) and MitoTracker (lower panels) in CTL and in UM 92.1 cells KD for macroH2A1.