Research Paper Volume 12, Issue 10 pp 9745—9760

Loss of macroH2A1 decreases mitochondrial metabolism and reduces the aggressiveness of uveal melanoma cells

Figure 5. KD for macroH2A1 reprograms energy metabolism in UM 92.1 cells. (A) mRNA levels MT-ND4, MT-CO2, COX4|1, MT-CYB, ATP5F1A and TFAM were assessed by qPCR, and normalized to GAPDH levels. Values are presented as mRNA relative levels and they are expressed as the mean ± SEM of three different experiments. (p < 0.0001 vs control). (B) mRNA levels NMNAT1, NMNAT2, SIRT1 and NAMPT. Values are presented as mRNA relative levels and they are expressed as the mean ± SEM of three different experiments. (**p<0.01****p < 0.0001 vs control). (C) Representative immunocytochemical images showing staining for TFAM (upper panels) and MitoTracker (lower panels) in CTL and in UM 92.1 cells KD for macroH2A1.