Research Paper Volume 12, Issue 10 pp 9781—9792

LncRNA DSCAM-AS1 promotes colorectal cancer progression by acting as a molecular sponge of miR-384 to modulate AKT3 expression

Figure 5. DSCAM-AS1 knockdown inhibited the progression of CRC cells by regulating miR-384/AKT3 axis. (A, B) The expression of AKT3 on mRNA and protein levels was measured in LOVO and HT29 cells after transfection with sh-NC, sh-DSCAM-AS1,sh-DSCAM-AS1+miR-384inhibitor(anti-miR-384)andsh-DSCAM-AS1+overexpression AKT3 plasmid(AKT3). (C). RT-PCR shows the AKT3 mRNA expression level in 56 pairs CRC tissues and non-tumor tissues. (D) Pearson's correlation analysis between AKT3 expression and DSCAM-AS1 expression in 56 CRC tissue. (E) Pearson's correlation analysis between AKT3 expression and miR-384 expression in 56 CRC tissue. (FH) Cell proliferation, migration and invasion were determined in LOVO and HT29 cells after transfection with sh-NC, sh-DSCAM-AS1, sh-DSCAM-AS1+anti-miR-384 and sh-DSCAM-AS1+AKT3. P < 0.05, **P < 0.01.