Research Paper Volume 12, Issue 10 pp 9793—9806

Circular RNA hsa_circ_0003141 promotes tumorigenesis of hepatocellular carcinoma via a miR-1827/UBAP2 axis

Figure 3. Downregulation of hsa_circ_0003141 inhibits proliferation of HCC cells. (A) The level of hsa_circ_0003141 in Huh-7 and Hep3B2.1-7 cells transfected with hsa_circ_0003141-shRNA1 or hsa_circ_0003141-shRNA2 analyzed by qRT-PCR. (B) Cell viability analyzed by CCK-8 assay in Huh-7 and (C) Hep3B2.1-7 cells transfected with hsa_circ_0003141-shRNA1 for 0, 24, 48 and 72 h. (D, E) Apoptosis measured by Annexin V and PI double staining in Huh-7 cells and (F, G) Hep3B2.1-7 cells transfected with hsa_circ_0003141-shRNA1 for 72 h. **P < 0.01, compared with the shRNA-NC group.