Figure 8. Knockdown of hsa_circ_0003141 inhibits tumorigenesis of Huh-7 subcutaneous xenografts in vivo. Mice were s.c. implanted with hsa_circ_0003141-shRNA1-transfected Huh-7 cells. (A) Xenograft tumor volume monitored weekly. (B, C) Xenografts tumors were photographed and calculated. (D) Expression of UBAP2 and cleaved caspase 3 in tumor tissues analyzed by western blotting. (E, F) The relative expression of UBAP2 and cleaved caspase 3 in tumor tissues normalized to β-actin. **P < 0.01, compared with the shRNA-NC group. ##P < 0.01, compared with the circRNA_shRNA1 group.