Research Paper Volume 12, Issue 10 pp 9807—9824

Identification and validation of hub microRNAs dysregulated in esophageal squamous cell carcinoma

Figure 1. Identification of differently expressed miRNAs (DEMs) in esophageal squamous cell carcinoma (ESCC). (A, B) GSE114110 data before and after normalization. (C, D) GSE43732 data before and after normalization. (E, F) Volcano plots of DEMs in GSE114110 and GSE43732, respectively. Black dots represent genes equally represented between ESCC and normal samples. Red and green dots represent upregulated and downregulated miRNAs, respectively. Volcano plots showing all DEMs. |log2FC| ≥ 1 and P < 0.05 were set as cut-off criteria. (G) Venn diagram analysis showing the top 10 upregulated and downregulated miRNAs in the two GEO datasets. (H) Identification of three upregulated and five downregulated miRNAs overlapping between both GEO datasets.