Research Paper Volume 12, Issue 10 pp 9935—9947

Gorlin syndrome-induced pluripotent stem cells form medulloblastoma with loss of heterozygosity in PTCH1

Figure 1. Generation of iPSCs from fibroblasts of patients with Gorlin syndrome. (A) Protocol for iPSC generation. (B) Phase-contrast microphotographs of Gln-iPSCs (G11, G12, G36, G72). (C) RT-PCR analysis of the Sendai virus. (D) Immunocytochemical analysis of Gln-iPSCs using antibodies to NANOG, OCT4/3, SOX2, SSEA4, and TRA1-60. (E) Expression of the endogenous TERT, NANOG, SOX2, OCT4/3, and DNMT3B genes. (F) in vitro differentiation of Gln-iPSCs into three germ layers. Immunocytochemical analysis of Gln-iPSCs using antibodies to Tuj-1, α-smooth muscle actin (SMA) and α-fetoprotein (AFP). Expression of the endogenous TERT, NANOG, SOX2, OCT4/3, and DNMT3B genes. (G) Karyotypes of Gln-iPSCs at the indicated passage number. Numbers in brackets indicate the number of cells analyzed.