Research Paper Advance Articles

Nrf2 inhibits ferroptosis and protects against acute lung injury due to intestinal ischemia reperfusion via regulating SLC7A11 and HO-1

Figure 2. Nrf2 regulates SLC7A11 and HO-1 to inhibit ferroptosis and protect against IIR-ALI. (A) Western blot analysis of Nrf2, HO-1, SLC7A11, and GPX4 expression in the lung tissues of each group. (B) The representative quantification of these proteins. (C) Relative mRNA expression of Nrf2, SLC7A11, and HO-1 in each group. (D) Representative transmission electron micrographs of the ultrastructure of the lung tissues. Scale bars: 1 μm. (E) Representative HE-stained lung sections. Morphology was examined using light microscopy. Scale bars: 200 μm. (F) Pathological scores were assigned by an experienced pathologist. (G) Western blot analysis of Nrf2, SLC7A11, and HO-1 expression in the lung tissues of each group. (H) The representative quantification of these proteins. (I) Relative mRNA expression of SLC7A11 and HO-1 in each group. (J) Western blot analysis of GPX4 expression in each group. (K) The representative quantification of GPX4 protein. (L) The lipid peroxide MDA level in each group. (M) The GSH level in each group. The error bars represent the standard error from three replicates. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.01;***P < 0.001. # compared with the IIR group in WT mice.