Research Paper Volume 12, Issue 13 pp 12943—12959

Nrf2 inhibits ferroptosis and protects against acute lung injury due to intestinal ischemia reperfusion via regulating SLC7A11 and HO-1

Figure 5. Low levels of SLC7A11 alleviate cell death by upregulating Nrf2-HO-1, whereas SLC7A11 overexpression (OE-SLC7A11) enhanced cell death. (A and C) Western blot analysis of the Nrf2, HO-1, and SLC7A11 in each group and the representative quantification of these proteins in MLE12 cells. (B and D) Cell viability was determined using a CCK-8 assay (n = 3). The error bars represent the standard error from three replicates. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.