Priority Research Paper Volume 12, Issue 10 pp 8766—8789

Muscle-dependent regulation of adipose tissue function in long-lived growth hormone-mutant mice

Figure 1. Effects of global deletion of Growth Hormone Receptor (GKO mouse) on the expression of UCP1 in adipose tissue. (A) Total RNAs were isolated from interscapular (brown fat), mesenteric, inguinal and perigonadal adipose tissues of 24-week-old wild type littermate control mice (WT) and GKO mice. mRNA levels of UCP1 (brown and beige fat marker) were measured by qRT-PCR. Data (mean ± SEM; n = 4) were normalized by the amount of GAPDH mRNA and expressed relative to the corresponding male WT value. *P < 0.05 versus WT. (B) Cell lysate was prepared from interscapular (brown fat), inguinal and perigonadal adipose tissues of 24-week-old WT and GKO mice. Protein levels of UCP1 (brown and beige fat marker) were then measured by western blotting. Representative gel images are shown. (C) Relative protein expression was normalized to β-actin levels. Values are mean ±SEM (n = 4).