Figure 5. Effects of muscle-specific deletion of GHR (MKO mice) on the expression of UCP1 in adipose tissue. (A) Total RNA was isolated from brown fat, mesenteric, inguinal and perigonadal adipose tissues of 24-week-old WT mice and MKO mice. mRNA levels of UCP1 were measured by qRT-PCR. Values were normalized by the amount of GAPDH mRNA and expressed relative to the corresponding male WT value. *P < 0.05 versus WT. (B) Cell lysate was isolated from interscapular (brown fat), inguinal and perigonadal adipose tissues of 24-week-old WT mice and MKO mice, and protein levels of UCP1 were measured by western blotting. Representative gel images are shown. (C) Relative protein expression was normalized to β-actin levels. Values are mean ±SEM (n = 4).