Priority Research Paper Volume 12, Issue 10 pp 8766—8789

Muscle-dependent regulation of adipose tissue function in long-lived growth hormone-mutant mice

Figure 6. Effects of global deletion of GHR (GKO mice) on adipose tissue macrophage infiltration and macrophage M1-M2 polarization. (A) Quantitative RT-PCR analysis of total RNA isolated from interscapular (brown fat), inguinal and perigonadal adipose tissues of 24-week-old WT and GKO mice for M1 macrophage markers (iNOS) and M2 macrophage markers (Arg1) mRNAs. Data (mean ± SEM; n = 4) were normalized by the amount of GAPDH mRNA and expressed relative to the corresponding male WT value. *P < 0.05, **P < 0.01 versus WT. (B) Cell lysate was isolated from interscapular (brown fat), inguinal and perigonadal adipose tissues of 24-week-old WT and GKO mice. The protein levels of iNOS and Arg1 were measured by western blotting. (C) Relative protein expression was normalized to β-actin levels. Values are mean ±SEM (n = 4).