Priority Research Paper Volume 12, Issue 10 pp 8766—8789

Muscle-dependent regulation of adipose tissue function in long-lived growth hormone-mutant mice

Figure 7. Adipose tissue macrophage infiltration and macrophage M1-M2 polarization of long-lived mice (DW and GKO). (A) Quantitative RT-PCR analysis of total RNA isolated from brown fat, inguinal and perigonadal adipose tissues of 24-week-old GKO mice and WT littermate mice for IL-6, TNFα, MCP-1 mRNAs. Values were normalized by the amount of GAPDH mRNA and expressed relative to the corresponding male WT value. *P < 0.05 versus WT. (B) Quantitative RT-PCR analysis of total RNA isolated from brown fat, inguinal and perigonadal adipose tissues of 24-week-old dw mice and WT mice for IL-6, TNFα, MCP-1 mRNAs. Data (mean ± SEM; n = 4) are expressed relative to the corresponding male WT value. *P < 0.05 versus WT.