Research Paper Volume 12, Issue 13 pp 12960—12986

LOXL2 from human amniotic mesenchymal stem cells accelerates wound epithelialization by promoting differentiation and migration of keratinocytes

Figure 2. Basic characterization of keratinocytes. (A) Representative phase-contrast bright field image (scale bar: 100 μm) shows a confluent culture of the human skin keratinocytes. (B) Fluorescence images (scale bar: 20 μm) show positive expression of the epithelial stem cell marker, Cytokeratin 19 (CK19; green) in the keratinocytes. The nuclei are stained with DAPI (blue). (C) Representative phase-contrast bright field image (scale bar: 100 μm) shows agglomerate morphology of keratinocytes when grown in medium containing 1.2mM Ca2+ for 7 days. (D) Histogram plots shows the relative mRNA levels of differentiation markers CK1 (Cytokeratin 1), CK10 (Cytokeratin 10), Involucrin and Filaggrin levels in the keratinocytes on days 0 and 7. Note: The values are expressed as means ±SEM; ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05.