Research Paper Volume 12, Issue 13 pp 13038—13058

circMET promotes NSCLC cell proliferation, metastasis, and immune evasion by regulating the miR-145-5p/CXCL3 axis

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Figure 6.

CXCL3 knockdown inhibited circMET-induced NSCLC progression in vitro. (A) and (B) Relative mRNA and protein expression of CXCL3 in several human NSCLC cell lines were examined by qRT-PCR (A) and Western blots (B). (C) The efficiency of transfection in the A549-circMET and 95D-circMET cell lines with CXCL3 shRNA plasmid were individually confirmed using qRT-PCR. GAPDH was used as a loading control. (D) Representative images of wound healing assay in CXCL3 knockout NSCLC cells with or without circMET overexpression. (E) and (F) Representative images of CCK-8 assay (E) and colony formation assay (F) in CXCL3 knockout NSCLC cells with or without circMET overexpression. (G) Representative images of the Matrigel transwell assay (G) in CXCL3 knockout NSCLC cells with or without circMET overexpression. Scale bar: 100um. The data are represented as the mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001.