Figure 7. Confocal images of microglia and astrocytes in the post-ischemic dorso-lateral nucleus of thalami (LD) of the rat brain. Fourfold immunofluorescence labeling microglia with Iba1 (green), astrocytes with GFAP (red), neurons with NeuN (yellow), and nuclei with DAPI (blue). The scale bar represents 50 μm. (A) Ctrl – control brain, (B) Isch – post-ischemic brain, (C) Quantification of the mean pixel intensities for Iba1 and GFAP signals of post-ischemic vs. control animals with 2 years survival. Values are presented as mean ± SEM. *** p<0.001. nCtrl = 18, nIsch = 18, n = number of analyzed cross sections. (D) Schematic representation of the rat hippocampus level with LD region indicated.