Research Paper Volume 12, Issue 13 pp 13220—13233

Cancer-associated fibroblasts-derived gamma-glutamyltransferase 5 promotes tumor growth and drug resistance in lung adenocarcinoma

Figure 5. High level of GGT5 in CAFs decreases the ROS level of LUAD cells. (A) The GGT activity in paired NFs and CAFs was tested in vitro (n = 6). (B) The level of GSH in NFs and CAFs or their conditioned media (NFs-M and CAFs-M) was detected. (C) The extracellular cysteine or glutamate in NFs-M and CAFs-M were analyzed respectively. (D) The relative intracellular GSH in A549 and LAC1 cells treated with different media: Normal-M, NFs-M or CAFs-M. (E) The relative intracellular ROS in A549 and LAC1 cells treated with different media. (F) The relative intracellular GSH in A549 and LAC1 cells treated with Vector-M or GGT5-M. (G) The relative intracellular ROS in A549 and LAC1 cells treated with Vector-M or GGT5-M. In all panels, data represent mean ± SEM. ns, no significant difference. *, P < 0.05; **, P < 0.01; ***, P < 0.001.