Research Paper Volume 12, Issue 13 pp 13234—13254

Increasing the expression of microRNA-126-5p in the temporal muscle can promote angiogenesis in the chronically ischemic brains of rats subjected to two-vessel occlusion plus encephalo-myo-synangiosis

Figure 3. Results of tube formation, scratch wound, and Transwell migration assays reflecting the functions of HUVEC. (A) Representative fields showing the results from the tube formation assays of each group. Bar = 100 μm. The results were quantified by (B) the number of tubes and (C) the average number of tube branch points. (D) Representative fields showing the results from the scratch wound assays at 48 h obtained for each group. Bar = 200 μm. (E) The results were quantified by the percentage of wound closure at 24 and 48 h. (F) Representative fields showing the results from the Transwell migration assays for each group. Bar = 50 μm. (G) The results were quantified by the invaded cells per field. The error bars represent the ± SDs.