Research Paper Volume 12, Issue 13 pp 13255—13280

Circular RNA circMBOAT2 promotes prostate cancer progression via a miR-1271-5p/mTOR axis

Figure 2. circMBOAT2 promotes cell proliferation, migration, and invasion in vitro. (A, B) qRT-PCR analysis of circMBOAT2 and MBOAT2 expression in PC-3 and DU145 cells treated with circMBOAT2 siRNAs. (C, D) CCK-8 assay determined the cell viability in PC-3 and DU145 cells treated with circMBOAT2 siRNAs. (E, F) Representative images and quantifications of colony formation assays in PC-3 and DU145 cells treated with circMBOAT2 siRNAs. (G, H) Representative images and quantifications of EdU assays in PC-3 and DU145 cells treated with circMBOAT2 siRNAs. Scale bars: 100 μm. (I, J) Representative images and quantifications of wound healing assays in PC-3 and DU145 cells treated with circMBOAT2 siRNAs. Scale bars: 200 μm. (K, L) Representative images and quantification of transwell assays in PC-3 and DU145 cells treated with circMBOAT2 siRNAs. Scale bars: 100 μm. Data are displayed as mean ± SD. *p < 0.05; **p < 0.01.