Research Paper Volume 12, Issue 13 pp 13365—13387

TCF7L2 rs290487 C allele aberrantly enhances hepatic gluconeogenesis through allele-specific changes in transcription and chromatin binding

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Figure 2.

The rs290487 variant modulates glucose metabolism in PLC-PRF-5 cells. (A) Schematic diagram showing protocol for the generation of PLC-PRF-5 cell line with rs290487 C/T point mutation using the CRISPR/Cas9 knock-in approach. (B) Glucose production is significantly higher in C/C cells compared to the C/T cells under fasting states. (C) Flow cytometry results show reduced 2NBDG uptake in insulin-stimulated C/C cells compared to the C/T cells under serum-free and glucose-free conditions. *: P< 0.05; **: P< 0.01.