Research Paper Volume 12, Issue 13 pp 13365—13387

TCF7L2 rs290487 C allele aberrantly enhances hepatic gluconeogenesis through allele-specific changes in transcription and chromatin binding

Figure 3. Basic characterization of PLC-PRF-5 cells with rs290487 C/C and rs290487C/T genotypes. (A) QRT-PCR analysis results show significant reduction in the expression of total TCF7L2 total mRNA and liver-specific alternative spliced transcripts containing exons 11-12-13, exons 11-13-14, and exon 8+15bp in the C/C cells compared to the C/T cells. (B) Dual luciferase reporter assay results show reduction in relative luciferase activity in cells transfected with pGL3-basic-promoter-rs290487 C/C transfected with pGL3-basic-promoter-rs290487 T/T. (C) Western blot results show that TCF7L2 protein levels are reduced in C/C cells compared to the C/T cells. (D) Western blot results show that TCF7L2 protein levels are increased in insulin- and high glucose- treated C/C and C/T cells. Note: * P< 0.05; ** P< 0.01.