Research Paper Volume 12, Issue 13 pp 13422—13436

Codonopsis pilosula polysaccharide attenuates Aβ toxicity and cognitive defects in APP/PS1 mice

Figure 7. CPPs attenuated the activity of active recombinant human β-Secretase 1 in vitro. (A) The activity of active recombinant human β-Secretase 1 (BACE1) was measured using β -Secretase Activity Fluorometric Assay Kit. Time curves for 32ng, 24ng and 16ng β-Secretase 1 were shown. Data were from three independent experiments. (B) 32ng active recombinant human β-Secretase 1 and indicated concentration of 250, 500, 1000 μg/ml CPPs were pre-incubated for one hour. BACE1 activity was measured. Data were from three independent experiments. P value significance is calculated from a one-way ANOVA or two-way ANOVA. **P < 0.01 vs. β-Secretase 1 without CPPs treatment.