Research Paper Volume 12, Issue 13 pp 13463—13476

Mechanistic study of mtROS-JNK-SOD2 signaling in bupivacaine-induced neuron oxidative stress

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Figure 4.

JNK signaling up-regulated of SOD2 transcription in BPV-induced neuron oxidative stress. (AF) the effect of JNK gene knock-down on the mtROS production, JNK phosphorylation and SOD2 transcription in cells treated with BPV. Con: cells transfected with silencer negative control siRNA; siJNK: cells transfected with JNK siRNA. BPV: cells transfected with silencer negative control siRNA and treated with 2.0 mM BPV for 60 min; siJNK+BPV: cells transfected with JNK siRNA and treated with 2.0 mM BPV for 60 min. (GJ) The effect of sp600125 on JNK activation and SOD2 transcription in cells treated with BPV; Con: untreated cells; SP: cells cultured with 10 μM sp600125 for 30 min; BPV: cells treated with 2.0 mM BPV for 60 min; SP+BPV: cells precultured with 10 μM sp600125 for 30 min, following treated with 2.0 mM BPV for 60 min. Values are the mean± SEM of n = 3, *: P< 0.05.