Research Paper Volume 12, Issue 13 pp 13488—13501

TRIM29 mediates lung squamous cell carcinoma cell metastasis by regulating autophagic degradation of E-cadherin

Figure 2. TRIM29 promotes metastasis and proliferation of lung squamous cell carcinoma cells in vitro. (A) Western blot analysis of TRIM29 expression in HNBE, HTB-182, CRL-5889, SK-MES-1, NCL-H520, and NCL-H1915. (B) Overexpresson of TRIM29 could significantly promote the proliferation of HTB-182 cells. (C) Knockdown of TRIM29 could significantly inhibit the proliferation of NCI-H1915 cells. (D) Colony formation analysis of TRIM29 over-expression treated HTB-182 cells. (E) Western blot analysis of cell proliferation-related biomarkers expression in TRIM29 over-expression treated HTB-182 cells. (F) Colony formation analysis of TRIM29 knockdown treated NCI-H1915 cells. (G) Western blot analysis of cell proliferation-related biomarkers expression in TRIM29 knockdown treated NCI-H1915 cells. (H) Migration and invasion analysis of TRIM29 over-expression treated HTB-182 cells. (I) Western blot analysis of EMT-related biomarkers expression in RIM29 over-expression treated HTB-182 cells. (J) Migration and invasion analysis of TRIM29 knockdown treated NCI-H1915 cells. (K) Western blot analysis of EMT-related biomarkers expression in knockdown treated NCI-H1915 cells. **P<0.01, ***P<0.001.