Research Paper Volume 12, Issue 13 pp 13488—13501

TRIM29 mediates lung squamous cell carcinoma cell metastasis by regulating autophagic degradation of E-cadherin

Figure 3. TRIM29 regulates autophagy degradation of E-cadherin. (A) Western blot analysis of TRIM29 and E-cadherin expression in HTB-182 cells with 0, 2, 4, 8 ug TRIM29 treatment. (B) Relative E-cadherin protein expression in HTB-182 cells with 0, 2, 4, 8 ug TRIM29 treatment. (C) Relative E-cadherin mRNA expression in HTB-182 cells with 0, 2, 4, 8 ug TRIM29 treatment. (D) Western blot analysis of TRIM29 and E-cadherin expression in TRIM29 over-expression treated HTB-182 cells with cyclohexane (CHX) treatment on different time points. (E) Relative E-cadherin protein expressions TRIM29 over-expression treated HTB-182 cells with cyclohexane (CHX) treatment on different time points. (F) Western blot analysis of TRIM29 and E-cadherin expression in TRIM29 over-expression treated HTB-182 cells with DMSO and MG132 treatment on different time points. (G) Relative E-cadherin protein expressions TRIM29 over-expression treated HTB-182 cells with DMSO and MG132 treatment on different time points. (H) Western blot analysis of TRIM29 and E-cadherin expression in TRIM29 over-expression treated HTB-182 cells with PBS and CQ treatment on different time points. (I) Relative E-cadherin protein expressions TRIM29 over-expression treated HTB-182 cells with PBS and CQ treatment on different time points. ***P<0.001.