Research Paper Volume 12, Issue 13 pp 13488—13501

TRIM29 mediates lung squamous cell carcinoma cell metastasis by regulating autophagic degradation of E-cadherin

Figure 4. TRIM29 promotes autophagy in lung squamous cell carcinoma. (A) Western blot analysis of TRIM29, LC3-I, and LC3-II in TRIM29 over-expression treated HTB-182 cells with or without CQ treatment. (B) Western blot analysis of TRIM29 and p62 in TRIM29 over-expression treated HTB-182 cells. (C) The relative account of LC3-II and p62 in TRIM29 over-expression treated HTB-182 cells. (D) confocal analysis of GFP-LC3II expression in TRIM29 over-expression treated HTB-182 with PBS and CQ treatment. (E) The relative GFP-LC3II expression in TRIM29 over-expression treated HTB-182 with or without CQ treatment. (F) Western blot analysis of TRIM29, LC3-I, and LC3-II in TRIM29 knockdown treated NCI-H1915 cells with or without CQ treatment. (G) Western blot analysis of TRIM29 and p62 in TRIM29 knockdown treated NCI-H1915 cells. (H) The relative account of LC3-II and p62 in TRIM29 knockdown treated NCI-H1915 cells. (I) confocal analysis of GFP-LC3II expression in TRIM29 knockdown treated NCI-H1915 cells with PBS and CQ treatment. (J) The relative GFP-LC3II expression in TRIM29 knockdown treated NCI-H1915 cells with or without CQ treatment. **P<0.01.