Research Paper Volume 12, Issue 13 pp 13488—13501

TRIM29 mediates lung squamous cell carcinoma cell metastasis by regulating autophagic degradation of E-cadherin

Figure 5. Inhibition of BECN1-induced autophagy leads to the disappearance of TRIM29-promoting phenotype. (A) qRT-PCR analysis of the TRIM29 and BECN1 expressions in TRIM29 over-expression treated HTB-182 cells. (B) qRT-PCR analysis of the TRIM29 and BECN1 expressions in TRIM29 knockdown treated NCI-H1915 cells. (C) Western blot analysis of TRIM29 and BECN1 expressions in TRIM29 over-expression treated HTB-182 cells and TRIM29 knockdown treated NCI-H1915 cells. (D) Western blot analysis BECN1, E-cadherin, and TRIM29 in TRIM29 over-expression treated HTB-182 cells with BECN1 knockdown treatment. (E) Migration and invasion analysis of HTB-182 cells with vector, TRIM29 over-expression, and TRIM29 over-expression+knockdown BECN1 treatment. (F) migration analysis of the number of HTB-182 cells with vector, TRIM29 over-expression, and TRIM29 over-expression+knockdown BECN1 treatment. (G) Invasion analysis of the number of HTB-182 cells with vector, TRIM29 over-expression, and TRIM29 over-expression+knockdown BECN1 treatment. **P<0.01, ***P<0.001.