Research Paper Volume 12, Issue 15 pp 15436—15445

MicroRNA-21-3p accelerates diabetic wound healing in mice by downregulating SPRY1

Figure 2. MiR-21-3p promotes fibroblast function in vitro. (A) The level of miR-21-3p in the control fibroblasts and the fibroblasts that were transfected with agomiR-21-3p or antagomiR-21-3p was measured by qRT-PCR; (B) The levels of cyclin D1 and cyclin D3 mRNA in the different groups were measured by qRT-PCR; (C) CCK-8 detected the proliferative ability of fibroblasts in the different groups; (D) The levels of Bcl-2 and Bax mRNA in the different groups were measured by qRT-PCR; (E) The levels of α-SMA, Col 1, and Col 3 mRNA in the different groups were measured by qRT-PCR; (F, G) The concentrations of bFGF and VEGF were measured by ELISA. Data are presented as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.