Research Paper Volume 12, Issue 15 pp 15436—15445

MicroRNA-21-3p accelerates diabetic wound healing in mice by downregulating SPRY1

Figure 4. Reduction of SPRY1 enhances fibroblast function. (A, B) The level of mRNA SPRY1 in the different groups was measured by qRT-PCR; (C) The levels of cyclin D1 and cyclin D3 mRNA in the different groups were measured by qRT-PCR; (D) CCK-8 assay detected the proliferative ability of fibroblasts in the different groups; (E) The levels of Bcl-2 and Bax mRNA in the different groups were measured by qRT-PCR; (F) The levels of α-SMA, Col 1, and Col 3 mRNA in the different groups were measured by qRT-PCR; (G, H) The concentrations of bFGF and VEGF were measured by ELISA. Data are presented as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.