Research Paper Volume 12, Issue 20 pp 20127—20138

Long non-coding RNA DLEUI promotes papillary thyroid carcinoma progression by sponging miR-421 and increasing ROCK1 expression

Figure 2. DLEU1 knockdown inhibits proliferation, migration and invasion of TPC-1 cells. (A) QRT-PCR analysis shows DLEU1 levels in sh-NC- and sh-DLEU1-transfected TPC-1 cells. (B) CCK8 assay results show proliferation rates of sh-NC- and sh-DLEU1-transfected TPC-1 cells. (C) Flow cytometry analysis shows the percentage apoptosis in sh-NC- and sh-DLEU1-transfected TPC-1 cells based on Annexin-V staining. (D) Wound healing assay results show the migration efficiency of sh-NC- and sh-DLEU1-transfected TPC-1 cells. (E) Transwell invasion assay results show the invasiveness of sh-NC- and sh-DLEU1-transfected TPC-1 cells. Note: The data is represented as the means ± SD of at least three independent experiments. *P< 0.05 and **P< 0.01.