Research Paper Volume 12, Issue 15 pp 15581—15602

hsa_circRNA6448-14 promotes carcinogenesis in esophageal squamous cell carcinoma

Figure 6. hsa-circRNA6448-14 promotes ESCC progression in cell lines. (A) Relative expression of hsa-circRNA6448-14 in ESCC lines and normal cell line by qRT-PCR. (B) hsa-circRNA6448-14 expression detected after transfection in KYSE-150 and TE-7 cells. (C) CCK-8 assays to determine viability of KYSE150 and TE7 cells. (D) Colony-formation assays to measure proliferation after hsa_circRNA6448-14 knockdown in KYSE-150 cells or overexpression in TE7cells. (E) Flow cytometry to detect apoptosis of KYSE150 and TE7 cells after transfection. (F) Wound healing assays to detect cell migration capacities of KYSE150 and TE7 cells after transfection. (G) Transwell assays to detect cell migration and invasion capacities of KYSE150 and TE7 cells after transfection. *p < 0.05, **p <0.01.