Research Paper Volume 13, Issue 4 pp 4976—4985

GPR173 agonist phoenixin 20 promotes osteoblastic differentiation of MC3T3-E1 cells

Agonism of GPR173 with phoenixin 20 promoted osteoblast differentiation. Cells were cultured with osteogenic medium (OM) with or without phoenixin 20 (15 nM) for 14 days. (A) Gene levels of ALP, OCN, Osx, and Col-I; (B) ALP activity; (C). Alizarin Red S staining (*, #, P

Figure 3. Agonism of GPR173 with phoenixin 20 promoted osteoblast differentiation. Cells were cultured with osteogenic medium (OM) with or without phoenixin 20 (15 nM) for 14 days. (A) Gene levels of ALP, OCN, Osx, and Col-I; (B) ALP activity; (C). Alizarin Red S staining (*, #, P<0.01, n=5).