Research Paper Volume 13, Issue 4 pp 4976—4985

GPR173 agonist phoenixin 20 promotes osteoblastic differentiation of MC3T3-E1 cells

The effects of Phoenixin 20 in osteoblast differentiation is mediated by p38. Cells were cultured in osteogenic medium containing phoenixin 20 (15 nM) with or without SB203580 (10μM) for 14 days. (A) Protein of RUNX-2; (B) Alizarin Red S staining; (C) ALP activity (*, #, P

Figure 6. The effects of Phoenixin 20 in osteoblast differentiation is mediated by p38. Cells were cultured in osteogenic medium containing phoenixin 20 (15 nM) with or without SB203580 (10μM) for 14 days. (A) Protein of RUNX-2; (B) Alizarin Red S staining; (C) ALP activity (*, #, P<0.01, n=4).