Research Paper Volume 12, Issue 19 pp 19147—19158

BRD4 inhibition sensitizes renal cell carcinoma cells to the PI3K/mTOR dual inhibitor VS-5584

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VS-5584 induces apoptosis activation in RCC 786-O cells. RCC 786-O cells were treated with applied concentrations of VS-5584 (0.5-10 μM), cells were further cultured for the indicated time; Cell death was tested by LDH medium release assay (A); Cell apoptosis was tested by ssDNA ELISA (B), Western blotting testing apoptosis proteins (C), and nuclei TUNEL staining (D). Data were presented as mean ± standard deviation (SD, n=5). *p< 0.05 vs.C” group. The in vitro experiments were repeated four times, and similar results were obtained.