Figure 1. circIQCH is upregulated in primary and metastatic breast cancer. (A) The expression level of five candidate circRNAs was validated in five paired primary and lung metastatic breast cancer tissues. (B) The relative expression of circIQCH in breast cancer tissues and adjacent normal tissues. (C) The relative expression of circIQCH in breast cancer cell lines. (D) RNase R assay confirmed the circular structure of circIQCH in SKBR3 cell line. (E) Circular transcripts of IQCH (circIQCH) was more stable than linear transcripts determined by Actinomycin D treated assay in SKBR3 cell line.