Research Paper Volume 12, Issue 19 pp 19233—19253
CircRNA CDR1as promotes hepatoblastoma proliferation and stemness by acting as a miR-7-5p sponge to upregulate KLF4 expression
Mir-7-5p affects the proliferation and stemness abilities of HB cells. (A–C) The cell proliferative ability of HB cells that were transfected with miR-7-5p was assessed by the Cell Counting Kit-8 (CCK-8), colony formation assay, and EdU assay; (D) The stemness of cancer stem cells (CSCs) was assessed using the sphere-forming assay after the HepG2 and HUH6 cells were transfected with miR-7-5p; (E) The proportion of CD133+ cells was determined by flow cytometric analysis after miR-7-5p was overexpressed in HB cells. Scale bar, 200 μm. Data are presented as the mean ± SEM of three experiments. *P < 0.05,**P < 0.01,***P < 0.001 (Student’s t-test).