Research Paper Volume 12, Issue 19 pp 19254—19272
S-allyl cysteine reduces osteoarthritis pathology in the tert-butyl hydroperoxide-treated chondrocytes and the destabilization of the medial meniscus model mice via the Nrf2 signaling pathway
SAC maintain extracellular matrix homeostasis in TBHP-treated chondrocytes. (A) Representative images and (B) Histogram plots show the levels of Aggrecan, ADAMTS5, MMP3 and MMP13 proteins in chondrocytes treated with or without SAC for 24 h and stimulated with 50 μM TBHP for 2 h. (C) Representative immunofluorescence images show COL2 expression in chondrocytes treated with or without SAC for 24 h and 50 μM TBHP for 2 h. The nuclei were stained with DAPI. Scale bar: 20 μm. (D) Histogram plots show the mean fluorescence intensity of COL2 as determined from the immunofluorescence images using the Image J software. Note: The data are presented as the means ± SD of three independent experiments; *p < 0.05, **p < 0.01, and ***p < 0.001.