Figure 2. Effects of AAV-Tim-3 treatment on Tim-3 protein levels and neurocyte apoptosis after SAH. Western blotting shows that AAV-Tim-3 significantly increased Tim-3 expression (A) and quantitative analysis of Tim-3 (B). Immunofluorescence staining also verified that AAV-Tim-3 significantly increased Tim-3 expression (C). Representative TUNEL staining images (D) and quantitative analysis of TUNEL-positive cells (E) in the ipsilateral cortex after SAH with AAV-Tim-3 or AAV-NC treatments; n = 6 in each group. Data are expressed as mean ± SD. **p < 0.01, ***p < 0.001.