Research Paper Volume 12, Issue 19 pp 19352—19364
CircPDZD8 promotes gastric cancer progression by regulating CHD9 via sponging miR-197-5p
CircPDZD8 upregulated the expression of CHD9 by modulating miR-197-5p in gastric cancer cells. (A–C) The mRNA and protein expression levels of CHD9 in SGC-7901 and MGC-803 cells transfected with si-NC, si-circPDZD8-1, si-circPDZD8-1 + inhibitor-NC or si-circPDZD8-1 + miR-197-5p-inhibitor were measured by RT-qPCR and western blot (MGC-803), respectively. (D) The proliferation of SGC-7901 and MGC-803 cells transfected with si-NC, si-circPDZD8-1, si-circPDZD8-1 + pcDNA3.1 or si-circPDZD8-1 + pcDNA3.1-CHD9 was examined by MTT assay. (E) The migration of SGC-7901 and MGC-803 cells transfected with si-NC, si-circPDZD8-1, si-circPDZD8-1 + pcDNA3.1 or si-circPDZD8-1 + pcDNA3.1-CHD9 was assessed by Transwell assay without Matrigel; *** P < 0.001.